Biological Properties of Experimental Methacrylate-Based Sealers Containing Calcium Phosphates

Abstract This study aimed to evaluate, in vitro and vivo, the biocompatibility of experimental methacrylate-based endodontic sealers containing α-tricalcium phosphate (α-TCP) or nanostructured hydroxyapatite (HAp). Experimental dual-cure with addition α-TCP HAp, at 10%wt were formulated compared AH Plus (AHP). Cell viability was assessed by 3-(4,5-dimethyl-thiazoyl)-2,5-diphenyl-tetrazolium bromide (MTT), sulforhodamine B (SRB). Sealers implanted rats’ subcutaneous tissue histologically evaluated. Bioactivity alkaline phosphatase enzyme activity (ALP) Alizarin Red (AR), using apical papillary cells (SCAPs), bone deposition measured surgical cavities on femur filled α-TCP. In both assays, HAp AHP similar, presented lower others MTT assay (p<0.05). A gradual decrease inflammatory response according periods observed only that giant (7-day period). Collagen fibers condensation increased periods, no differences among sealers. There an increase ALP mineralized nodules periods. higher values for 5 days 5, 10, 15 AR different from superior 10 At 90 days, control (empty cavity) showed high a sealer, bioactivity, potential be used as clinical practice. Further investigations are required gain information physicochemical properties these formulation before its implementation.